Journal: Translational Cancer Research

Article Title: Constructing a chemokine-based model and identifying CCL17 as a core biomarker associated with immune infiltrates in thyroid cancer

doi: 10.21037/tcr-2024-2654

Figure Lengend Snippet: The mRNA and protein expression of hub genes. (A) The mRNA expression of hub genes in normal and THCA samples. (B) The mRNA expression of hub genes in low- and high-risk groups. (C) The expression of hub genes in TPC-1 and Htori-3 cell lines. Representative immunohistochemistry images of (D) ACKR3 , (E) CCL2 , and (F) CCL17 in both normal and THCA tissues sourced from the Human Protein Atlas database ( https://www.proteinatlas.org/ ). Image credit goes to the Human Protein Atlas. The links to the individual normal and tumor tissues of each protein are provided for ACKR3 ( https://www.proteinatlas.org/ENSG00000144476- ACKR3 /tissue/thyroid+gland#img, https://www.proteinatlas.org/ENSG00000144476-ACKR3/cancer/thyroid+cancer#img ), CCL2 ( https://www.proteinatlas.org/ENSG00000108691-CCL2/tissue/thyroid+gland#img , https://www.proteinatlas.org/ENSG00000108691-CCL2/cancer/thyroid+cancer#img ), and CCL17 ( https://www.proteinatlas.org/ENSG00000102970-CCL17/tissue/thyroid+gland#img , https://www.proteinatlas.org/ENSG00000102970-CCL17/cancer/thyroid+cancer#img ), respectively. Scale bar, 100 µm. **, P<0.01; ***, P<0.001. mRNA, messenger ribonucleic acid; THCA, thyroid cancer.

Article Snippet: Healthy human thyroid cell (Htori-3, CL-0817) and THCA cell (TPC-1, CL-0643) were acquired from Procell (Wuhan, China).

Techniques: Expressing, Immunohistochemistry

Journal: Translational Cancer Research

Article Title: Constructing a chemokine-based model and identifying CCL17 as a core biomarker associated with immune infiltrates in thyroid cancer

doi: 10.21037/tcr-2024-2654

Figure Lengend Snippet: The mRNA and protein expression of hub genes. (A) The mRNA expression of hub genes in normal and THCA samples. (B) The mRNA expression of hub genes in low- and high-risk groups. (C) The expression of hub genes in TPC-1 and Htori-3 cell lines. Representative immunohistochemistry images of (D) ACKR3 , (E) CCL2 , and (F) CCL17 in both normal and THCA tissues sourced from the Human Protein Atlas database ( https://www.proteinatlas.org/ ). Image credit goes to the Human Protein Atlas. The links to the individual normal and tumor tissues of each protein are provided for ACKR3 ( https://www.proteinatlas.org/ENSG00000144476- ACKR3 /tissue/thyroid+gland#img, https://www.proteinatlas.org/ENSG00000144476-ACKR3/cancer/thyroid+cancer#img ), CCL2 ( https://www.proteinatlas.org/ENSG00000108691-CCL2/tissue/thyroid+gland#img , https://www.proteinatlas.org/ENSG00000108691-CCL2/cancer/thyroid+cancer#img ), and CCL17 ( https://www.proteinatlas.org/ENSG00000102970-CCL17/tissue/thyroid+gland#img , https://www.proteinatlas.org/ENSG00000102970-CCL17/cancer/thyroid+cancer#img ), respectively. Scale bar, 100 µm. **, P<0.01; ***, P<0.001. mRNA, messenger ribonucleic acid; THCA, thyroid cancer.

Article Snippet: Healthy human thyroid cell (Htori-3, CL-0817) and THCA cell (TPC-1, CL-0643) were acquired from Procell (Wuhan, China).

Techniques: Expressing, Immunohistochemistry

Journal: Discover oncology

Article Title: PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway.

doi: 10.1007/s12672-024-01596-8

Figure Lengend Snippet: Fig. 1 PTEN was downregulated in THCA cells. A: GEPIA database was used to analyze the expression of PTEN in THCA tumor tissues; Red box plots indicated the Tumor group (n = 512); Gray box plots represented the Normal group (n = 337); Log2(TPM + 1) served as the unit of gene expression in the Y axis; B: ENCORI database was used to analyze the PTEN expression in THCA tumor tissues; C–D: RT-qPCR and West- ern blot were employed to confirm mRNA and protein levels of PTEN in normal thyroid follicular epithelial cells Nthy-ori 3–1 and THCA cells B-CPAP, FTC-133, and TPC-1, respectively. Data were expressed as mean ± SD, and cellular experiments were repeated three times indepen- dently. One-way analysis of variance was utilized for comparison among multiple groups, * P < 0.05, *** P < 0.001

Article Snippet: Normal human thyroid follicular epithelial cells Nthy-ori 3–1 (CL-0817) and human THCA cell lines B-CPAP (CL-0575), FTC-133 (CL-0644) and TPC-1 (CL-0643) were provided by Procell (Wuhan, Hubei, China).

Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Comparison

Journal: Discover oncology

Article Title: PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway.

doi: 10.1007/s12672-024-01596-8

Figure Lengend Snippet: Fig. 2 Overexpression of PTEN suppressed proliferation and EMT and promoted apoptosis of THCA cells. The oe-PTEN and oe-NC were transfected into TPC-1 cells. After 48-h transfection, A: RT-qPCR was carried out to determine mRNA levels of PTEN; B: Western blot assay was implemented to determine protein levels of PTEN, p-AKT1 and AKT1; Cells were cultured for an additional 72 h, C: Cell viability was measured by CCK-8; D: Flow cytometry was utilized to estimate apoptosis; E: Protein levels of epithelial marker E-cadherin and mesenchy- mal markers N-cadherin and Vimentin were assessed by Western blot. Data were expressed as mean ± SD. The cellular experiments were repeated three times independently, One-way analysis of variance was used for comparison among multiple groups, ** P < 0.01, *** P < 0.001

Article Snippet: Normal human thyroid follicular epithelial cells Nthy-ori 3–1 (CL-0817) and human THCA cell lines B-CPAP (CL-0575), FTC-133 (CL-0644) and TPC-1 (CL-0643) were provided by Procell (Wuhan, Hubei, China).

Techniques: Over Expression, Transfection, Quantitative RT-PCR, Western Blot, Cell Culture, CCK-8 Assay, Flow Cytometry, Marker, Comparison

Journal: Discover oncology

Article Title: PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway.

doi: 10.1007/s12672-024-01596-8

Figure Lengend Snippet: Fig. 3 Invasion and migration of THCA cells were inhibited by overexpression of PTEN. A: Transwell assay for cell migration and invasion ability; B: Scratch assay for cell wound-healing ability. Data were expressed as mean ± SD, and the cellular experiments were repeated three times independently. Comparison among multiple groups was conducted by one-way analysis of variance. ** P < 0.01

Article Snippet: Normal human thyroid follicular epithelial cells Nthy-ori 3–1 (CL-0817) and human THCA cell lines B-CPAP (CL-0575), FTC-133 (CL-0644) and TPC-1 (CL-0643) were provided by Procell (Wuhan, Hubei, China).

Techniques: Migration, Over Expression, Transwell Assay, Wound Healing Assay, Comparison

Journal: Discover oncology

Article Title: PTEN inhibits epithelial mesenchymal transition of thyroid cancer cells by regulating the Wnt/β-Catenin signaling pathway.

doi: 10.1007/s12672-024-01596-8

Figure Lengend Snippet: Fig. 5 The inhibitory effect of PTEN overexpression on malignant behavior of THCA cells was partially reversed by Wnt activator. TPC-1 cells were transfected with oe-PTEN plasmid. After transfection for 48 h, cells were treated with Wnt activator BML-284 (0.5 μM) for 24 h. A: West- ern blot assay to determine levels of β-catenin and c-myc; Cells were cultured for an additional 72 h, B: CCK-8 assay to detect cell viability; C: Flow cytometry to examine apoptosis; D: Western blot assay to assess protein levels of E-cadherin, N-cadherin and Vimentin; E: Transwell assay to test cell migrating and invasive capacities; F: Scratch assay to evaluate cell wound-healing ability. Data were reported as mean ± SD and the cellular experiments were repeated three times independently. One-way analysis of variance was used for comparison among mul- tiple groups, * P < 0.05, ** P < 0.01

Article Snippet: Normal human thyroid follicular epithelial cells Nthy-ori 3–1 (CL-0817) and human THCA cell lines B-CPAP (CL-0575), FTC-133 (CL-0644) and TPC-1 (CL-0643) were provided by Procell (Wuhan, Hubei, China).

Techniques: Over Expression, Transfection, Plasmid Preparation, Cell Culture, CCK-8 Assay, Flow Cytometry, Western Blot, Transwell Assay, Wound Healing Assay, Comparison